The third section of the course examines transcription and its regulation.  We are going to initially look at prokaryotic transcription and use both the Lac Operon and Lambda Phage to explore the precise regulation of gene expression.  The readings for each section are outlined below with links to the specific web pages.  There is also a link to the online review page for this exam, practice exam problems and their answers.

Exam III Review

Exam III Practice Problems: Exam 3 Practice.pdf

Exam III Practice Problem Answers:Exam 3 Practice Answers.pdf

Topic 10 Prokaryotic Transcriptional Machinery

Readings:

• Molecular Biology of the Cell: From DNA to RNA, Promoter Consensus Sequence Figure, Transcription Cycle Figure

Animations:

• Transcription: From Sigma Factor to Rho-dependent Termination
• Prokayotic versus Eukaryotic: mRNA Processing

Topic 11-12 The Genetic Switch by Mark Ptashne

Topic 13 The Lac Operon

Readings and Animations:

• A wonderful flash animation of the Lac Operon